Optimization of extraction process of flaxseed oil by orthogonal design
Flaxseed, also known as flaxseed, is the dry mature seed of Linum Usitatissimum L. Flaxseed contains 35 to 45% of , which is a high oil content substance. The content of unsaturated fatty acids in linseed oil was 86.04%, while linolenic acid content was 49.05%. Alpha-linolenic acid (ALA) is an essential fatty acid for human body. It can synthesize eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) through desaturation and carbon chain elongation. ALA, EPA and DHA have the functions of developing intelligence, protecting eyesight, lowering blood pressure, lowering blood lipid, anti-thrombosis, delaying aging, anti-allergy and preventing heart disease. Muscle infarction has broad application prospects in medicine, health care products and other fields.
Ultrasound extraction is a new method developed in recent years. It has the advantages of short extraction time, simple operation and energy saving. With the development of research field, ultrasonic technology has entered the oil industry, and its cavitation effect can be used to separate and extract oil in industrial production. In this experiment, the optimum extraction technology of flaxseed oil extractor was analyzed and established by using ultrasonic extraction technology, which could provide theoretical basis for further separation and purification of flaxseed oil.
Flaxseed is screened, mildewed flaxseed is removed, dried at 55 C, crushed and sifted through 40 meshes for reserve. The water content of flaxseed powder was determined by constant weight method at 105. 36% and 2.2. The extraction method accurately weighed 10.00g of flaxseed powder and put it into 250 ml conical bottle. Petroleum ether was used as solvent to extract the flaxseed powder by ultrasonic-assisted technology. Each group of experiments was extracted three times to save solvent. New petroleum ether was used for the first and second time, and the third was used to extract the flaxseed powder. Secondary recovery of petroleum ether, combined with three extracts, filtrate with rotary evaporator vacuum recovery of petroleum ether, that is, flaxseed oil. Weigh them separately and calculate the oil rate.
Ultrasound assisted extraction technology can effectively reduce the production cost and improve the extraction rate. In this experiment, four main factors affecting the ultrasonic extraction of flaxseed oil were studied, and the orthogonal experiment was used to optimize the extraction process. Finally, the four factors affecting the extraction power > liquid-to-material ratio > temperature > time were obtained. The results of variance analysis showed that there were significant differences in influencing factors of extraction power. The optimum extraction conditions were A3B2C2D2, i.e. liquid-solid ratio 1:10, temperature 50 C, time 30 min, power 280w. The process has good repeatability and saves time. It can be used for the scientific research and production conditions of further separation and purification of flaxseed oil.